for mouse behavioral training
This system is automated platform with voluntary head-restraint system for mouse operant training, which is supervised by Dr. Andrea Benucci (Laboratory for Neural Circuits and Behavior, RIKEN Center for Brain Science, Japan).
Subject animals learn operant tasks with stress-free under voluntary self head-fixation. This platform will contribute automated high-throughput data collection with physiological device.
Reference : Aoki, R., Tsubota, T., Goya, Y., Benucci, A. (2017) An automated platform for high-throughput mouse behavior and physiology with voluntary head-fixation. Nature Comms., 8: 1196
learning & memory
The subject is put in the cylindrical applicator type restraint chamber made of the stainless steel. A head of the subject is fixed with special metal fittings, so that only eyes, forelegs and a mouth can be freely moved.
A horizontally moving constant torque lever with integrated water spout at the tip is placed in front of subject. This type of lever is called as spout lever. Using foreleg subject can operated the spout lever by push or pull. Encoder present in the spout lever outputs lever movement.
Spout lever movement range can be divided into GOAL/FORWARD/BACKWARD areas. During trial, based on the position of spout lever, decision can be made for correctness and liquid reward dispensed thorough the water spout present at the tip of lever. Various types of stimuli like sound stimuli/ visual stimuli/ odor stimuli/ intracranial photo stimulation can be presented. All the presented stimuli are program controllable. Varieties of schedules are available by software. Cables and speakers of this system are electromagnetically-shielded. This enables this system to be used with physiological recording system.
1.Water supply unit OPR-7300
2.Sound stimulation unit OPR-8210
4.Spout lever unit OPR-SPL-RM
5.Acclimation applicator OPR-TUBE-R
6.Rat restrainer OPR-Rφ60
|1 Water supply unit
|2 Sound stimulation unit
|4 Spout lever unit
|5 Acclimation applicator
|6 Rat restrainer
|7 Task control software
|8 Computer set
learning & memory
Visuals (both static image and animation) can be displayed through LCD panel and touch response recorded through the special application software. Based on the touch response reward (pellet or liquid) can be provided.
It is possible to construct and execute variety of tasks by combining different visuals, cue sound, reward type and response. User can execute both simple operant task and complex tasks such as discrimination task, matching to sample task. It is also possible to evaluate impulsivity of subject animal.
Two kinds of data files are automatically saved as text file, one is per trial result file and the other is event log file.
1.Touch panel operant chamber for mouse
4.Water supply unit (pump & bottle)
5.Water supply unit (water supply sonde & fixing tool)
6.Drawer mount table
8.LED house light
|1 Touch panel operant chamber for mouse
|3 Pellet dispenser
|4 Water supply unit (pump & bottle)
|5 Water supply unit (water supply sonde & fixing tool)
|6 Drawer mount table
|7 Ventilation fan
|8 LED house light
learning & memory
※Multi-chamber system (1 – 8 chamber)
In the operant test, the conditioned stimulus is applied and then reinforcement is provided by the response of lever-pressing, poking, licking, or a touch panel. We can configure the operant test system for your experimental purpose, budget, and any other request.